Astrocytes and catalase prevent the toxicity of catecholamines to oligodendrocytes
Identifieur interne : 004276 ( Main/Exploration ); précédent : 004275; suivant : 004277Astrocytes and catalase prevent the toxicity of catecholamines to oligodendrocytes
Auteurs : Paul G. Noble [Canada] ; Jack P. Antel [Canada] ; Voon Wee Yong [Canada]Source :
- Brain Research [ 0006-8993 ] ; 1994.
English descriptors
Abstract
Abstract: Metabolism of catecholamines can generate reactive free radical species, including hydrogen peroxide (H2O2), that are potentially harmful to cells. In this study, norepinephrine (NE) and epinephrine (EPI) were found to be toxic oligodendrocyte (OL) cultures derived from adult rat brain. The catecholamine toxicity, reproduced by equimolar concentrations of H2O2, could be completely prevented by simultaneous treatment of OLs with the H2O2-decomposing enzyme catalase. These results implicate H2O2 produced by metabolism of NE and EPI as the toxic intermediate. Since OLs in vivo are not normally susceptible to the toxicity of catecholamine neurotransmitter molecules, we sought to examine the involvement of another cell type closely apposed to OL, that is astrocytes, as a protectant against catecholamine toxicity. When adult rat OLs were seeded onto a monolayer of neonatal rat astrocytes, the toxicity of NE, EPI and H2O2 to OLs was completely prevented; medium conditioned by astrocytes did not prevent the manifestation of H2O2 toxicity on OLs. We conclude that the OL-myelin complex is vulnerable to free radical-mediated damage, especially when the protective functions of astrocytes are impaired.
Url:
DOI: 10.1016/0006-8993(94)91525-3
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 002068
- to stream Istex, to step Curation: 002068
- to stream Istex, to step Checkpoint: 001C03
- to stream Main, to step Merge: 004A33
- to stream Main, to step Curation: 004276
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Astrocytes and catalase prevent the toxicity of catecholamines to oligodendrocytes</title><author><name sortKey="Noble, Paul G" sort="Noble, Paul G" uniqKey="Noble P" first="Paul G." last="Noble">Paul G. Noble</name></author><author><name sortKey="Antel, Jack P" sort="Antel, Jack P" uniqKey="Antel J" first="Jack P." last="Antel">Jack P. Antel</name></author><author><name sortKey="Yong, Voon Wee" sort="Yong, Voon Wee" uniqKey="Yong V" first="Voon Wee" last="Yong">Voon Wee Yong</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:ECC85FAC93172CF5073442E2A22D00BDDF9C8C87</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1016/0006-8993(94)91525-3</idno><idno type="url">https://api-v5.istex.fr/document/ECC85FAC93172CF5073442E2A22D00BDDF9C8C87/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">002068</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">002068</idno><idno type="wicri:Area/Istex/Curation">002068</idno><idno type="wicri:Area/Istex/Checkpoint">001C03</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001C03</idno><idno type="wicri:doubleKey">0006-8993:1994:Noble P:astrocytes:and:catalase</idno><idno type="wicri:Area/Main/Merge">004A33</idno><idno type="wicri:Area/Main/Curation">004276</idno><idno type="wicri:Area/Main/Exploration">004276</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Astrocytes and catalase prevent the toxicity of catecholamines to oligodendrocytes</title><author><name sortKey="Noble, Paul G" sort="Noble, Paul G" uniqKey="Noble P" first="Paul G." last="Noble">Paul G. Noble</name><affiliation wicri:level="4"><country>Canada</country><wicri:regionArea>Montreal Neurological Institute, McGill University, 3801 University Street, Montreal, Que.</wicri:regionArea><orgName type="university">Université McGill</orgName><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Antel, Jack P" sort="Antel, Jack P" uniqKey="Antel J" first="Jack P." last="Antel">Jack P. Antel</name><affiliation wicri:level="4"><country>Canada</country><wicri:regionArea>Montreal Neurological Institute, McGill University, 3801 University Street, Montreal, Que.</wicri:regionArea><orgName type="university">Université McGill</orgName><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Yong, Voon Wee" sort="Yong, Voon Wee" uniqKey="Yong V" first="Voon Wee" last="Yong">Voon Wee Yong</name><affiliation wicri:level="4"><country>Canada</country><wicri:regionArea>Montreal Neurological Institute, McGill University, 3801 University Street, Montreal, Que.</wicri:regionArea><orgName type="university">Université McGill</orgName><placeName><settlement type="city">Montréal</settlement><region type="state">Québec</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Brain Research</title><title level="j" type="abbrev">BRES</title><idno type="ISSN">0006-8993</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">633</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="83">83</biblScope><biblScope unit="page" to="90">90</biblScope></imprint><idno type="ISSN">0006-8993</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-8993</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Astrocyte</term><term>Catalase</term><term>Cytotoxicity</term><term>Free radical</term><term>Multiple sclerosis</term><term>Oligodendrocyte</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Metabolism of catecholamines can generate reactive free radical species, including hydrogen peroxide (H2O2), that are potentially harmful to cells. In this study, norepinephrine (NE) and epinephrine (EPI) were found to be toxic oligodendrocyte (OL) cultures derived from adult rat brain. The catecholamine toxicity, reproduced by equimolar concentrations of H2O2, could be completely prevented by simultaneous treatment of OLs with the H2O2-decomposing enzyme catalase. These results implicate H2O2 produced by metabolism of NE and EPI as the toxic intermediate. Since OLs in vivo are not normally susceptible to the toxicity of catecholamine neurotransmitter molecules, we sought to examine the involvement of another cell type closely apposed to OL, that is astrocytes, as a protectant against catecholamine toxicity. When adult rat OLs were seeded onto a monolayer of neonatal rat astrocytes, the toxicity of NE, EPI and H2O2 to OLs was completely prevented; medium conditioned by astrocytes did not prevent the manifestation of H2O2 toxicity on OLs. We conclude that the OL-myelin complex is vulnerable to free radical-mediated damage, especially when the protective functions of astrocytes are impaired.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Québec</li></region><settlement><li>Montréal</li></settlement><orgName><li>Université McGill</li></orgName></list><tree><country name="Canada"><region name="Québec"><name sortKey="Noble, Paul G" sort="Noble, Paul G" uniqKey="Noble P" first="Paul G." last="Noble">Paul G. Noble</name></region><name sortKey="Antel, Jack P" sort="Antel, Jack P" uniqKey="Antel J" first="Jack P." last="Antel">Jack P. Antel</name><name sortKey="Yong, Voon Wee" sort="Yong, Voon Wee" uniqKey="Yong V" first="Voon Wee" last="Yong">Voon Wee Yong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Canada/explor/ParkinsonCanadaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004276 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004276 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Canada
|area= ParkinsonCanadaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:ECC85FAC93172CF5073442E2A22D00BDDF9C8C87
|texte= Astrocytes and catalase prevent the toxicity of catecholamines to oligodendrocytes
}}
| This area was generated with Dilib version V0.6.29. |  |